Immunophenotypic characterization of acute leukemias of ambiguous lineage- new diagnostic entity of the revised WHO classification 2008

Česká verze

Authors: I. Marinov ^1,2; D. Mikulenková ¹; A. Luxová ¹; V. Tkáčová ¹
Authors place of work: Ústav hematologie a krevní transfuze ¹; Ústav klinické a experimentální hematologie ÚHKT a 1. LF UK, Praha ²
Published in the journal: Transfuze Hematol. dnes,16, 2010, No. 3, p. 166-171.
Category: Souhrnné práce, původní práce, kazuistiky

Summary

Immunophenotyping of leukemic cells by polychromatic flow cytometry is playing an important role in the diagnosis, classification, follow-up of acute leukemias and crucial role in the management of acute leukemias of ambiguous lineage. In the revised WHO classification 2008 the former acute biphenotypic leukemia was renamed mixed phenotype acute leukemia (MPAL) and new definition criteria were established. The aim of the new classification was to simplify existing EGIL scoring system of acute biphenotypic leukemia, to assign lineage as lymphoid or myeloid if possible and to include both biphenotypic and bilineage types. The goal of this article is to approach the new aspects of immunophenotypic characterization of acute leukemias of ambiguous lineage in the context of our own experience.

Key words:
immunophenotyping, flow cytometry, acute leukemias of ambiguous lineage, revised WHO classification 2008

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