Separation of whole blood and gravity-based manufacturing of blood components by the disable set ErySet^® with a hollow-fibre filter

Česká verze

Authors: M. Bohoněk; M. Petráš; T. Hrádek; B. Kostrouchová; E. Sládková; M. Kricnerová; K. Rollerová; V. Mašková; S. Duchková
Authors place of work: Oddělení hematologie, biochemie a krevní transfuze, Ústřední vojenská nemocnice – Vojenská fakultní nemocnice Praha
Published in the journal: Transfuze Hematol. dnes,19, 2013, No. 2, p. 88-95.
Category: Souhrnné práce, původní práce, kazuistiky

Summary

The article describes the method of whole blood separation using hollow-fibre filters and the manufacture of leukodepleted red blood cells and plasma using only the force of gravity. It also evaluates the parameters of manufactured blood product quality. The hollow-fibre filter is an integral part of the disposable ErySep^® (LMB GmbH, Germany) set, which includes a leukodepletion filter for whole blood filtration. Results from two validation studies are presented. The first study compared the quality parameters of products manufactured using the disposable ErySep^® set with the conventional method for manufacturing leukodepleted RBCs and plasma using the classical centrifugation method (Macopharma disposable set Leucoflex with in-line RBCs leukofilter). The second study focused on the quality parameters of blood products manufactured by the new generation of ErySep^® disposable set. The results confirm that blood components manufactured using the ErySep^® hollow-fibre system have comparable parameters to products manufactured using the standard method. Red cell recovery is even higher. The tested disposable set is suitable for manufacturing high-quality blood products without the additional need for any expensive machines, such as centrifuges, blood separators, etc. This set could be most beneficial for medical facilities where appropriate technical equipment is not available such as field hospitals and in places (or countries) lacking modern infrastructure.

Key words:
separation of whole blood, hollow-fibre filter

Zdroje

1. Sekiguchi S, Takahashi TA, Yamamoto S, et al. A new type of blood component collector: plasma separation using gravity without any electrical devices. Vox Sang 1990; 58 : 182–187.

2. Schwarzfischer G, Heim MU, Bock M, Kratzer MA, Mempel W. Combination of a simple hollow fibre system with leukocyte filter for production of leukocyte depleted erythrocyte concentrates and plasma. Beiträge zur Infusionstherapie und Transfusionsmedizin 1992; 30 : 287-291.

3. Schwarzfischer G, Heim M, Mempel W. Der Einsatzeines einfachen Hohlfasersystems zur Auftrennung von präoperativen Eigenblutspenden. Hämatologie München, Sympomed 1992; 1 : 108–113.

4. Eichler H, Kretschmer V. Component removal from whole blood by means of gravity sedimentation. Beiträge zur Infusionstherapie und Transfusionsmedizin 1994; 32 : 12-15.

5. Brune T, Hannemann-Pohl K, Nissle K, Ecker N, Garritsen H. Quality, Stability, and Safety Data of Packed Red Cells and Plasma Processed by Gravity Separation Using a New Fully Integrated Hollow-Fibre Filter Device. Hindawi Publishing Corporation, Advances in Hematology 2009, Article ID 175234, 6 pages.

6. Stienstra S, Hannemann-Pohl K, Hornsey VS, Meierling H, Heim G. Results of blood separation without centrifuge steps with the new Sangofer blood separation device. Transfusion 2005; 45S: 123.

7. Hornsey VS, McColl K, Drummond O, Prowse CV. Separation of whole blood into plasma and red cells by using a hollow-fibre filtration system. 16^st Regional Congress of the ISBT. Vox Sang 2005; 89 : 81–85.

8. Brune T, Fill S, Heim G, Rabsilber A, Wohlfarth K, Garritsen HSP. Quality and stability of red cells derived from gravity-separated placental blood with a hollow-fiber system, Transfusion 2007; 47 : 2271–75.

9. Bohoněk M, Kostrouchová B, Petráš M, Hrádek T, Staropražská V, Duchková S. Quality evaluation of blood components manufactured by using a hollow-fibre separation technology. 31^st International Congress of the ISBT, Berlin, SRN. Vox Sang 2010; 99S: 18.

10. Bohoněk M, Kricnerová M, Sládková E, Hrádek T, Petráš M. Quality Parameters of Red Cells and Plasma Manufactured by Hollow-Fibre Separation, 32^nd International Congress of the ISBT. Vox Sang 2012; 103S: 39-4C-S30-02.

11. Bohoněk M. Blood Components Manufactured by Using a Hollow-Fibre Separation Technology. Transfusion Days of Serbia 2012, Niš, Serbia. Bulletin of Transfusion Medicine 2012; 58 : 27-28.