Influence of processing of platelet lysate at selected of platelets factors

Česká verze

Authors: B. Kubešová
Authors place of work: Tkáňové zařízení ; Kryo s. r. o.
Published in the journal: Transfuze Hematol. dnes,19, 2013, No. 4, p. 223-228.
Category: Souhrnné práce, původní práce, kazuistiky

Summary

The author focused on factors from platelet alpha granules, specifically the PDGF, IGF-1, a VEGF and TGF. Platelets were first broken by deep frozen that had washed factors into the plasma. Plasma was heat-treated, thereby leading to precipitation of coagulation factors, as well as to inactivate the complement. After centrifugation platelet lysate was pushed to the bag. Riboflavin solution was added and platelet lysate was viral and bacterial inactivated by Mirasol system. The final solution was freeze-dried.

The author of validated each critical step. Platelet factors were measured at the beginning of the process, following the inactivation of microbial and after freeze-dried. The production process had not a significant impact on the level of platelet factors. This finding is crucial for the other using of platelet lysate.

Key words:
platelet factor, growth factor, platelet lysate, platelet rich plasma

Zdroje

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